After 96 h of culture, cells were harvested and analyzed by flow cytometry to acquire the proliferative information of SNARF-1 negative and CFSE positive staining responder cells. ModFit TM software was then used to get more proliferation related index.

• 混合培养96h后收获细胞,用流式细胞术进行检测,分析SNARF-1-CFSE+应答细胞的增殖情况,并用ModFitTM软件拟和以获得更多增殖相关指数。