After 2 hours reperfusion, infarct size and risk region were measured by postmortem dual dyes with triphenyl tetrazolium chloride (TTC) and Evans blue.

• 家兔处死后以Evans蓝及氯化三苯基四氮唑 (TTC)双重染色确定缺血及梗死心肌范围 ,心肌梗死范围以梗死心肌占危险区心肌重量百分比 (MI/RISK)表示。