Adsorpted Calmodulin could not be eluted by EQTA or high salt solution and it was eluted by 50% ethanol. This results indi cate that the hydrophobic binding to the Polymer is independent of calcium induced hydrophobic interaction on Calmodulin.

 
  • 钙调蛋白在3520吸附树脂上的选择性吸附作用不依赖于钙离子的存在,EGTA或高浓度的盐溶液均不能洗脱,但可被50%25乙醇洗脱,此结果表明钙调蛋白与吸附树脂的结合为疏水结合,与钙离子诱导的疏水区变化无关。
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