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- The Pseudorabies virus (PRV) Fa strain gC gene was subcloned into Adenovirus shuttle vector pDC316 through double enzymatic digestion. 通过双酶切将伪狂犬病毒(Pseudorabies virus,PRV)Fa株gC囊膜糖蛋白基因片段亚克隆到5型腺病毒AdMax系统穿梭质粒pDC316中,得到pDC316-gC。
- Adenovirus shuttle vector 腺病毒5型穿梭载体
- We also have constructed two shuttle vector plw9,psc11m1 which can recombine to FPV genome. 我们还构建了可以和FPV基因组发生重组的穿梭载体，plw9和psc11。
- The recombinant shuttle vector pBacPAK-CTB and pBacPAK-4aa-CTB was co-infected with Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened, plaque-purified and identified by PCR and Southern hybridization. 重组转移载体DNA与经Bsu36I酶切线性化的修饰型病毒Bm-BacPAK6 DNA共转染家蚕BmN细胞，然后经空斑纯化、PCR扩增、Southern杂交等方法鉴定出含CTB基因的重组病毒rBm-BacCTB和rBm-Bac4aa-CTB。
- As the pMCLacI/neo shuttle vector is a novel bi-functional mutation detection system, apart from be used in above transgenic animal study , it was also used in our study of MNNG-indued DNA damage and repair research. 由于本研究率先在突变检测系统中应用了具有双功能的pMCLacI/neo穿梭载体,在利用转基因动物技术建立突变检测系统及展开相关研究的同时，我们又利用基因敲除技术，定向敲除DNA损伤修复酶基因-MGMT，建立了一系列MGMT基因定向敲除的突变检测系统，及高表达MGMT的突变检测系统，并展开了相应的DNA损伤修复的研究。
- The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in ?cis? and ?trans? direction under T4 promoter, separetely. 切下目的片段与酶切穿梭质粒pJFF224-XN连接,经PCR及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下。
- The target fragments were cut down from pGEMT-easy and ligated with shuttle vector pJFF224-XN/E. coRI. Through identification, the homogenous regulatory gene inserted in cis and trans direction under T4 promoter, separately. 切下目的片段与酶切的穿梭质粒pJFF224-XN连接,经PCR方法及酶切鉴定,调节基因片段分别以正向或反向插入到质粒的T4启动子下;
- Then, the DNA fragment xylR-gfp which were obtained after pGEM-xylRGFP being digested by Kpn I and Sph I , was inserted into Kcoli-B.subtilis shuttle vector pRP22, and the resulted recombinant plasmid was named at pRP22-GFP. 经SphI/KpnI双切质粒pGEM-xylRGFP后，回收片段xylR-gfp，插入穿梭载体pRP22，得到重组子pRP22-GFP。
- E. coli-B. subtilis shuttle vector 穿梭载体
- E.coli-mycobacterium shuttle vector 大肠杆菌-分枝杆菌穿梭质粒
- The strain BSG is more efficient recipient of transformation for shuttle vectors pPTE4 and pBE2 from E. 来自大肠杆菌的穿梭质粒在BSG菌中转化频率较亲本高。
- shuttle vector of adenovirus 重组腺病毒穿梭载体
- Construction of an EB Virus Based Shuttle Vector Plasmid pZH32 穿梭质粒pZH32的构建
- The U.S. made the first space shuttle in the world. 美国制造了世界上第一架航天飞机。
- The space shuttle will orbit a weather satellite. 太空梭将把一个气象卫星送入运行轨道。
- I'm flying to Boston on the shuttle. 我将乘穿梭班机去波士顿。
- The buses shuttle visitors to Disneyland. 区间公共汽车将游客载至迪尼斯乐园。
- Construction and Optimization of E. Coli and B.Subtilis Shuttle Vector 大肠杆菌枯草杆菌穿梭载体的构建及优化
- Construction of Shuttle Vector by Using Thya Gene as a Selective Pressure 以胸苷酸合成酶基因为选择压力的穿梭质粒载体的构建
- The shuttle moves backwards and forwards through the warp. 梭子在经线中来回移动。
