Add anti-Annexin V FITC and PI staining, examination apoptotic and necrotic rate by flow cytometer. Early apoptotic cells present Annexin+/PI-, late apoptotic and necrotic cells present Annexin+/PI+ and normal cells presentAnnexin-/PI-, respectively.

• 流式细胞术ANNEXIN V－FITC加碘化丙陡（PI）双染检测细胞凋亡率和坏死率，早期凋亡细胞为Annexin”/PI，晚期凋亡和继发坏死细胞Annexin”/PI，正常活细胞为 Annexin/PI。