According to published sequence of the New York strain of West Nile virus, one pair of unique primers for WNV E protein domain III were designed, and the domain III gene of WNV was amplified by polymerase chain reaction (PCR).

• 根据已发表的序列,设计合成一对针对NY99株西尼罗河病毒E蛋白结构域III基因序列的特异性引物,以引进的含WNV E蛋白基因的重组质粒为模板,用聚合酶链式反应(PCR)扩增WNV E蛋白结构域III的基因,大小为360bp。