According the these genes sequence of MVV(Accession Number: AY101611), 4 pair of primers were designed, and 4 fragments were obtained and sequenced by PCR amplification from the whole viral genome of infected cells extracted by the method of Proteinase K.

• 方法:根据Genbank中发表的绵羊梅迪-维斯纳病毒美国株85/34的基因组序列(检索号:AY101611)设计合成了4对引物,用蛋白酶K法提取MVV前病毒DNA,对主要基因片段进行PCR扩增,并对扩增产物进行了克隆和测序。