AIM:To eliminate the influence of serum on self-renewal of embryonic stem cells(ESCs),knockout serum replacement(KSR),a defined formulation,was used to replace serum for the establishment of C57BL/6J mouse ESC line.

• 目的:在培养液中添加knockout血清替代品(knockout serum replacement,KSR)代替胎牛血清(FBS)用于建立C57BL/6J小鼠胚胎干细胞(ESC)细胞系,以便消除血清中的不确定因子对ESC增殖的影响。