A phospholipase C (PLC) was successfully purified by ammonium sulfate precipitation followed by a application of ion-exchange chromatography (DEAE-Sepharose Fast Flow) and gel filtration (Sephadex G-100 chromatography).

• 先后采用(NH4)2SO4分级盐析、DEAE-Sepharose Fast Flow阴离子交换层析、Sephadex G-100凝胶过滤、再次DEAE-Sepharose Fast Flow阴离子交换层析等方法从哈维氏弧菌SF-1菌株的上清液中分离纯化得到磷脂酶C。