A new assay for indirectly detecting nitric oxide in serum was established. Nitrate was reduced to nitrite by activated spongy cadmium with copper ion on a microscale chromatographic column, then the nitrite was measured by the Griess reaction.

• 采用铜离子活化海绵镉微型柱层析法将血清中的硝酸盐 (NO-3 )还原为亚硝酸盐 (NO-2 ) ；再通过Griess反应测定NO-3 /NO-2 总量以反应体内一氧化氮 (NO)水平 ；从而建立血清NO间接测定新方法 .