A monoclonal antibody specific for TG-Ab has been pre-coated onto a microplate.Standards and samples are pipetted into the wells and any TG-Ab present is bound by the immobilized antibody.

• 用纯化的抗体包被微孔板，制成固相抗体，往包被抗体的微孔中依次加入TG-Ab、生物素化的抗人TG-Ab抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。