A monoclonal antibody specific for IFN-a Ab has been pre-coated onto a microplate.Standards and samples are pipetted into the wells and any IFN-a Ab present is bound by the immobilized antibody.

• 用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IFN-a Ab抗原、生物素化的抗人IFN-a Ab抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。