A PCR fragment (550bp) from defoliating pathotype of Verticillium dahliaewas produced with a couple of specific primers, but not from nondefoliating pathotype of Verticillium dahliae.

• 用一对棉花落叶型黄萎病菌的特异性引物D1和D2进行PCR扩增 ,对于落叶型黄萎病菌 ,该对引物可特异性地扩增产生一段 550bp的产物 ,而非落叶型黄萎病菌则不能被扩增。