A HPLC method was developed for determination of acetaminophen (1)solution. A reverse-phase C 18 column was used and the mobile Phase was methanol-0.05 mol/L ammonium acetate (15:85). The internal standard was theophylline(2).

• 采用HPLC法以十八烷基硅烷键合硅胶为固定相，甲醇－0.；05mol/L醋酸铵溶液（15：85）为液动相，茶碱为内标，测定对乙酰氨基酚溶液的含量。