A Dot-ELISA on nitrocellulose menbrane as solid-phase carrier was developed for detection of 284 hybridoma supernatants for the presence of monoclonal antibodies agaist Beet mosaic virus (BMV), among which 80 positive samples were screened.

• 确立了以硝酸纤维素膜(NCM)作固相载体的Dot-ELISA的最适试验程序；并用此技术检测了284个杂交瘤培养上清；筛选出80个分泌抗甜菜花叶病毒(Beet mo(>)ic virus)抗体的上清样品；阳性率达28.；17%25。