A 220 bp segment encoding rmBD2 was amplified by RT-PCR and proved by sequencing. The SiHa/K cells transfected pcDNA3.1(+) and SiHa had no expression of rmBD2 protein.

• 提取稳定转染4、6、8、10周SiHa/rmBD2细胞总RNA,RT-PCR反应扩增得到220bp左右的目的片段,而SiHa/K及SiHa细胞未见目的蛋白表达。