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- PCR requires the synthesis of oligonucleotide primers complementary to the target DNA. The entire sequence of the target DNA does not need to be known, only the flanking regions. 就整个PCR扩增技术而言，引物的设计占有十分重要的地位，它的优劣直接关系到PCR的特异性与成功与否。
- Based on the study, it is very convenient for making a deep research of the foreign gene expression under the regulation of CSN2 flanking region. 为进一步利用CSN2质粒两端的调控序列, 插入新的基因, 研究其表达功能, 或者进行乳腺生物反应器的研究奠定了基础。
- Methods: 201 type 2 diabetics, 4 idiopathic type 1 diabetics and 100 control subjects were included in the study.Two exons of IPF-1(including the coding and flanking regions) were amplified using PCR. 方法：选择2型糖尿病201例、特发性1型糖尿病4例及正常对照组100例作为研究对象，对IPF-1基因的两个外显子序列（包括编码区及侧翼区）进行PCR扩增，PCR产物经纯化后，经ABI 377 DNA 全自动测序仪对所有PCR产物直接测序。
- Cloning and sequence analysis of 5'flanking region of prolactin gene in Small Tail Han sheep 小尾寒羊催乳素基因5'侧翼调控区的克隆与序列分析
- Positional identification of transcription regulation elements in 5'flanking region of NGAL gene NGAL基因5'侧翼区转录调控元件的分段定位鉴定
- 5 'flanking region 5’侧翼序列
- These fish vary in weight from 3 lb to 5 lb. 这些鱼的重量从3磅到5磅不等。
- The going rate for freelance work is 5 an hour. 自由职业者的现行酬金是每小时5英镑。
- 5' flanking region 5’侧翼区
- Many children under 5 go to nursery school. 5岁以下的许多儿童都上幼儿园。
- Meals average out at 5 per head. 膳食平均每人5英镑。
- Children under 5 can travel for nothing. 5岁以下儿童可免付旅费。
- Comparative analysis of genetic variations of pgm locus and its flanking regions in Yersinia pestis 鼠疫耶尔森菌pgm位点及其侧翼序列遗传变异的比较分析
- Even the cheapest was priced at 5. 即使最便宜的也标价5英镑。
- The liquid was 5 litres in volume. 该液体的体积为5升。
- The ratios of 1 to 5 and 20 to 100 are the same. 1与5之比和20与100之比相同。
- The winning time was 1 minute 5 seconds. 获胜的时间是1分零5秒。
- I found a 5 note on the pavement. 我在人行道上捡到了一张5英镑的纸币。
- He is 5 centimeters taller than her. 他比她高5公分。
- He is 5 centimetres taller than her. 他比她高5公分。
