3) Purified recombinant Ad plasmid DNA is digested with PacI to expose its inverted terminal repests( ITR), and then used to transfect AD-293 cells where deleted viral assembly genes are complemented in vivo.

 
  • 3) 用线性化的重组腺病毒转染293细胞,获得基因组结构均一的重组腺病毒。
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