2 Difference of the IVF rate between frozen-thawed sperm and fresh spermFrozen-thawed mouse sperm were fertilized with nude oocytes or oocytes wrapped with cumulus oophorus and matrix (COM).

• 冻融过程造成部分小鼠精子结构破坏和功能障碍，能够使卵受精的精子也发生实验小鼠精子低温保存及体外受精的研究了某些变化，使冻融精子体外受精(IVF)与新鲜精子有所不同。