16 primers out of 99 were screened out and 135 ISSR markers were amplified from these clones with 114 polymorphic loci.

• 实验从99条引物中筛选出多态性较高的16条ISSR引物进行PCR扩增；共扩增出135个位点；其中多态性位点数为114个；多态性位点比率为84.；44%25。