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- 自H5N1阳性病料中提取总RNA,反转录后采用高可信度DNA聚合酶,经PCR扩增NP基因,采用Invitrogen定向表达系统进行克隆表达,纯化获得N末端携带多聚组氨酸标签的重组NP蛋白,分子质量约66.0ku。Total RNA was extracted from positive clinical sample of H5N1 subtype of avian influenza virus, NP gene was amplified by reverse transcriptase-polymerase chain reaction using high proofreading polymerase (PyobestTM DNA Polymerase), and expressed using Invitrogen championTM pET directional TOPO expression system. Recombinant protein containing polyhistidine (6xHis) tag in N-terminal was about 66.0 ku in size, had been obtained and purified.
- 多聚组氨酸融合蛋白histidinetagged protein
- 带有组氨酸标签的马传染性贫血病毒感染性分子克隆的构建Generation of a histidine-tagged equine infectious anemia virus
- 目的用DNA重组技术在毕赤酵母中表达多聚组氨酸融合重组乙型肝炎病毒表面抗原S蛋白,与血源乙型肝炎Dane颗粒联合使用,制备乙型肝炎表面抗体酶免试剂盒。Objective To study on the expression of His-Tagged S protein of hepatitis B virus in Pichia pastoris and its application in combination with the blood-origin hepatitis B virus Dane particles for immunoassay of HBsAb.
- 组氨酸标签histidine tag
- 聚组氨酸Poly ( L - histidine)
- 纯化组氨酸标签蛋白金属螯合亲和色谱填料的制备与性能Preparation of metal chelate affinity chromatographic material and its application in purification of histidine-tagged protein
- 多聚甘氨酸polyglycine
- 聚组氨酸镍复合膜polyhistidine - Ni film
- ε-多聚赖氨酸ε - polylysine
- 多聚左旋赖氨酸Poly-L-Lysine
- 多聚赖氨酸修饰Decoration with poly--L--lysine
- β-丙氨酰组氨酸β-alanylhistidine
- 多聚丙氨酸延展polyalanine expansion
- 脆性组氨酸三联体基因在大肠癌中的表达及同预后的关系Expression of Fragile Histidine Triad in Colorectal Carcinoma and its Relation to Prognosis
- 高分子量激肽原富含组氨酸区域抑制细胞伸展的机制分析Inhibition of Cell Spreading by Recombinant Histidine-rich Domain of High Molecular Weight Kininogen and Its Mechanism
- 多聚赖氨酸硅藻土polylysine-kieselguhr
- 铜-组氨酸copper histidine
- 多聚乙酰神经氨酸colominic acid
- 组氨酸酶histidase