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- 根据Calnek疫苗株序列设计了9对引物,利用反转录聚合酶链式反应,成功地扩增了AEV-NH937毒株的全基因。The second part was seguence analysis of the genome of the AEV-NH937.Nine pairs of primers were designed according to published Calnek vaccine strain of AEV. RT-PCR was used to amplify the cDNA of the genome .
- 物matter
- 引物primer
- 反anti-
- 引to lead
- 用反转录-聚合酶链反应快速鉴别新城疫病毒Rapid molecular differentiation of virulent and avirulent strains of Newcastle disease virus with RT-PCR technique
- 用反转录PCR(RT-PCR)检测转染细胞ATF基因的表达;Then the expression of ATF was analyzed by means of reverse transcription polymerase chain reaction (RT-PCR).
- 猪内源性反转录病毒5'端非编码区的克隆及结构分析Cloning of Porcine Endogenous Retrovirus 5'-Untranslated Region and Analysis of its Structure
- 简并引物degenerate primer
- 诱变引物mutagenic primer
- 电化学发光反转录聚合酶链式反应技术检测齿兰环斑病毒Electrochemiluminescence Reverse Transcription Polymerase Chain Reaction Detection of Odntoglossum Ringspot Virus in Orchids
- 因而,设计PCR引物时宜选择Takahashi等分离株为标准。In China these viruses share high homology with Takahaski’s strains which might be good standards for primer designation.
- 反转录-PCRRT-PCR
- 外引物outer primer
- 巢式反转录PCRNested RT-PCR
- 引物股primer strand
- 反转录巢式PCRRT-nested PCR
- 引物链primer strand
- 反转录病毒载体介导人血管内皮生长因子基因治疗实验性兔脑缺血Retroviral vector mediated vascular endothelial growth factor gene therapy for experimental rabbits with cerebral ischemia
- 引物酶primase