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- 采用Trizol试剂对病毒进行了核酸提取,电泳结果发现有4条核酸条带,分别为3.0kb、1.3kb、0.8kb、0.75kb,对RNA核酸酶、S1核酸酶敏感,对DNA酶抵抗,为单链RNA病毒。The nucleic acid of virus was extracted by using the Trizol reagents and analyzed with 1%25 agarose electrophorosis. It shows the nucleic acid has four bands sized 3.0, 1.3, 0.8 and 0.75 kb respectively, and is sensitive to RNase and S1 nuclerase and resistant to DNase, indicating these bands as single?strand RNA.
- 背景和目的：博尔纳病病毒（Borna disease virus, BDV）是一种有套膜的非分节段的单分子负链RNA病毒。Background and Objective:Borna disease virus (BDV) is an enveloped, nonsegmented, mononegative RNA virus.
- 双链RNA病毒诱导的牙鲆胚胎细胞差减cDNA文库的构建Construction of a Subtractive cDNA Library from the Paralichthys olivaceus Embryonic Cells Induced by a Double-Stranded RNA Virus
- 病毒virus
- 单链载体single-stranded vector
- RNA病毒载体RNA viral vectors
- 双链RNA表达dsRNA expression
- 植物RNA病毒载体构建策略The Strategy for Constructing Plant RNA Virus Vector
- 短链RNA干扰short interfering RNA
- 单链RNAsingle-stranded RNA
- 双链RNA分解酶dsRNA dependent ribonuclease
- 动物RNA病毒载体对外源基因的表达Foreign genes expression by using animal RNA virus based expression vectors
- 单链线single catenary
- 单链噬菌体single stranded phage
- 单链DNAsingle-stranded DNA; ssDNA
- 双链RNA激活的蛋白激酶(PKR)的克隆表达及其对丙型肝炎病毒蛋白合成的抑制作用Cloning and Expression of Double-Stranded RNA Activated Protein Kinase(PKR) and Its Effect on Hepatitis C Virus Internal Ribosome Entry Site(IRES) Directed Protein Synthesis
- 单链环single-stranded loop
- 双链RNA依赖的蛋白激酶(KR)脊椎动物中由干扰素介导的抗病毒防御机制的重要组成部分。The double-stranded- RNA-dependent protein kinase( PKR) is an important component in an antiviral defence pathway that is mediated by interferon( IFN) in vertebrates.
- 单链泡single-strand bubble
- 单链Fabsingle chain Fab
