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- DEAE-Cellulose-32DEAE-Cellulose-32
- benzy-cellulose (光学塑料)苯基纤维素
- 平菇萃取液经酸性沉淀、热变性、Phenyl-Sepharose CL-4B疏水亲和层析和DEAE-Cellulose 52离子交?Q层析分冉出了电泳纯的真菌钙调素。Mushroom calmodulin (CAM) proved to be electrophoretic homogeneity is obtained in extract of pleurotus ostreatus hymenophore by means of acidic isoelectric point precipitation, heating treatment, Phenyl-Sepharose CL-4B hydrophobic chromatography and DEAE-Cellulose 52 ion exchange chromatography.
- 菜心粗蛋白和DEAE-Cellulose收集的GO作SDS-PAGE,并对GO抗体作western blot,结果显示,粗蛋白有75 kD、61kD、49kD等印迹带其中49kD为主带。Results of western blot analysis of GO from Brassica parachinensis Bailey at various purification steps, using the mouse antiserum raised against GO, shew the main immunity band of crude proteins was 49kD, but the bands of 75kD, 61kD were also clear.
- 用pH提取、硫酸胺沉淀、AmberliteIRC 5 0及DEAE Cellulose柱层析等方法 ,从羊垂体提纯制备了羊黄体生成素 ,并测定了分子量和未端氨基酸 .Applying such methods as pH extraction, ammonium sulphate precipitate, chromatography on Amberlite IRC 50 and on DEAE Cellulose, etc, Ovine pituitary LH is prepared and purified from sheep hypophysis and its molecular weight and terminal amino are determined.
- 菜心粗蛋白、35%(NH_4)_2SO_4沉淀、过SephadexG-50柱收集到的部分纯化GO以及过DEAE-Cellulose柱收集到的纯化GO作SDS-PAGE,并对40kD亚基抗体作western blot。Western blot analysis of GO from Brassica parachinensis Bailey atvarious purification steps after separation by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), mouse antiserum raised against 40kD subunit was used, shew that result of purified GO was different from those of partly purified GO.
- 细菌纤维素(Bacterial cellulose,简称BC)是由生长在液态含糖基质中的细菌产生的,并分泌到基质中的纤维素成分,它不是细菌细胞壁的结构成分,而是一种胞外产物。Some bacteria such as Acetobacter produces cellulose in liquid culture, thecellulose produced by bacteria is called bacteria cellulose or bio-cellulose.
- 用葡聚糖凝胶Sephadex G-200柱层析法、三氯醋酸-乙醇提取物DEAE-Cellulose(DE52)柱层析法、尿素提取物Sepharose 4B柱层析法对华支睾吸虫成虫可溶性抗原进行部分纯化,获得8种不同的抗原活性组分.Crude adult worm antigen (AWA) of Clonorchis sinensis was purified by three different procedures respectively: 1.AWA pass through sephadex G-200, 2. trichloroacetic acid soluble alcohol insoluble composition pass through DEAE- cellulose (DE-52), 3. 8M urea soluble composition pass through sepharose 4B. 8 different purified antigen fractions were obtained.