[methods]1 To insert inverted terminal repeats(ITR) of adeno - associated virus(AAV) intopORF5-mIL-10 plasmid and to construct the murine interleutin-10 plasmid enhanced by ITR.

• 1 利用分子克隆技术，将AAV中的ITR插在pORF5-mIL-10质粒启动子的上游，构建pORF-ITR-mIL-10质粒。